Amazing Spider-Man #6 (2004)

When reports of a humanoid-lizard (naturally dubbed "The Lizard") come out of Florida), Daily Bugle_(Earth-616)) publisher J. Jonah Jameson) issues a challenge to Spider-Man): Defeat the Lizard). Hearing about this, Peter goes to see what Jameson's intentions are. He learns that Jameson only issued the challenge to sell more papers.

Later, while at the Natural History Museum with his classmates, Peter takes an express interest in the lizard exhibits. When crooks try to rob the museum and take Liz Allan), hostage, Peter slips away and changes into Spider-Man and comes to her rescue. Hearing another report of an attack by the Lizard in Florida, Peter decides to take Jameson's challenge. As Spider-Man, Peter pays Jameson a visit to take him up on his challenge, in the hopes that he'd send Peter Parker to Florida to take pictures of the event. The plot works, however, it backfires ever so slightly: Jameson himself is going to accompany the boy in order to supervise him.

When they arrive in Florida, Peter makes an excuse of needing to buy film for his camera to get away from Jameson. As Spider-Man, Peter checks out his only lead: Curtis Connors, a resident expert on lizards. However, when he arrives at the Connors home, Spider-Man learns from Curt's wife that her husband is the Lizard: He was trying to find a way to allow humans to grow back limbs. Since Connors had lost his arm in the war, he used himself as a guinea pig on the project. While his arm grew back, the side effects of the serum he created caused him to transform into the Lizard.